2 edition of Structural features of rat goblet cell mucin. found in the catalog.
Structural features of rat goblet cell mucin.
Raafat Emil Fahmy Fahim
Written in English
|The Physical Object|
|Number of Pages||235|
Mucinous colorectal cancers exhibit a characteristic set of molecular genetic alterations and may be derived from progenitor cells committed to the goblet cell lineage. Previously, we demonstrated that the MUC2 mucin gene promoter drives transgene reporter expression with high specificity in small intestinal goblet cells of transgenic mice. On the basis of these experiments, we reasoned that. (C) Thin-section histologic images of rat colons are shown (original magnification ×20): (CT) colon of CT-W rat in which numerous goblet cells show Muc2 immunoreactivity; (PO) photomicrograph of a PO rat colon showing enormous goblet cells; (RO) colon of a rat administered RO; and (SO) colon of a rat administered SO. Goblet-cell hyperplasia is a critical pathological feature in hypersecretory diseases of airways. However, the underlying mechanisms are unknown, and no effective therapy exists. Here we show that stimulation of epidermal growth factor receptors (EGF-R) by its ligands, EGF and transforming growth factor α (TGFα), causes MUC5AC expression in airway epithelial cells both in in vitro and in vivo. A: immunohistochemistry of paraffin-embedded rat intestinal tissue after incubation with anti-rat intestinal mucin antiserum. This section is oriented with the intestinal lumen at top. Immunolabeling is seen as a brown reaction product. Arrows and arrowhead indicate examples of immunoreactive goblet cells and mucus layer, respectively.
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Fahim RE, Forstner GG, Forstner JF. Heterogeneity of rat goblet-cell mucin before and after reduction. Biochem J. Jan 1; (1)– [PMC free article] Filipe MI, Fenger C. Histochemical characteristics of mucins in the small intestine. A comparative study of normal mucosa, benign epithelial tumours and by: Goblet cell mucin (GCM) of rat small intestine has been isolated previously, and its location established by immunofluorescence techniques.
In the present study, GCM was characterized more fully by chemical and physical by: HARALD MOE, The Goblet Cells, Paneth Cells, and Basal Structural features of rat goblet cell mucin. book Cells of the Epithelium of the Intestine, /B, (), (). Crossref Tamotsu Iwai, Fine structure and absorption patterns of intestinal epithelial cells in rainbow trout alevins, Zeitschrift f r Zellforschung und Mikroskopische Anatomie, 10 Cited by: The intestinal goblet cells secrete not only the MUC2 mucin but also a number of typical mucus components: CLCA1, FCGBP, AGR2, ZG16, and TFF3.
The goblet cells have recently been shown to have a novel gate‐keeping role for the presentation of oral antigens to the immune by: Europe PMC is an archive of life sciences journal literature.
Abstract. With the use of a newly developed solid-phase radioimmunoassay method, the major antigenic determinants of human small-intestinal goblet-cell mucin were investigated and related to the overall tertiary structure of the by: Goblet-cell mucin of rat small intestine was purified from mucosal scrapings by using centrifugation, Sepharose 4B and Sepharose 2B chromatography.
The mucin was applied in low concentrations (1 microgram/track) to slab gels containing % agarose/2% (w/v) polyacrylamide, and bands were detected after electrophoresis by silver stain or by.
General Structural Features. Complete amino acid sequences have been described for frog (Xenopus) integumentary mucins FIM-A.1 and FIM-B.1 (), PSM (), RSM (), MSM (), MUC2 (), MUC5B (), and MUC7 and almost complete sequences for MUC5AC and rat Muc2 ().The different domains of mucins are shown in FigMany of the domains show sequence identities and possibly similar functions in.
Summary Pectin enhances mucin secretion in the rat small intestine. However, what structural features of pectin to stimulate mucin secretion remain unclear. The study aimed to clarify active constituents of pectin using a human goblet cell line, HTMTX.
Various pectins at mg/L commonly stimulated MUC5AC secretion, irrespective of their differ. The major macromolecular components of mucus, the mucin glycoproteins, are secreted by surface epithelial goblet cells and submucosal gland mucous and serous cells or are tethered to cell membranes.
1 The cell surface-associated mucins attached to airway epithelial microvilli and cilia generate an osmotic barrier that preserves the periciliary. Depletion of colonic mucus occurs before invasion of the colonic mucosa by Entamoeba histolytica trophozoites.
It is hypothesized that E. histolytica releases a mucus secretagogue; this Structural features of rat goblet cell mucin. book studied in a rat colonic loop model.
In colonic loops exposed to live amebae, mucus secretion was quantitated by release of acid-precipitable [3H]glucosamine-labeled luminal glycoprotein and by specific. Peter Baluk, Jay A. Nadel, Donald M. McDonald, Calcitonin Gene-related Peptide in Secretory Granules of Serous Cells in the Rat Tracheal Epithelium, American Journal of Respiratory Cell and Molecular Biology, /ajrcmb/, 8, 4, (), ().
LaMont JT, Ventola AS. Purification and composition of colonic epithelial mucin. Biochim Biophys Acta. Nov 20; (1)– Mantle M, Forstner GG, Forstner JF. Antigenic and structural features of goblet-cell mucin of human small intestine. Biochem J. Jan 1; (1)– [PMC free article] Miller HR. The mucus layer coating the gastrointestinal tract is the front line of innate host defense, largely because of the secretory products of intestinal goblet cells.
Goblet cells synthesize secretory mucin glycoproteins (MUC2) and bioactive molecules such as epithelial membrane-bound mucins (MUC1, MUC3, MUC17), trefoil factor peptides (TFF), resistin-like molecule β (RELMβ), and Fc-γ.
Mucociliary clearance is a major function of the airway epithelium. This important function depends both on the physicochemical properties of the airway mucus and on the activity of the cilia.
The former, in turn, is dependent mainly on the quality and quantity of mucous glycoproteins or mucins, which are produced by two different cell types, namely, goblet cells of the epithelium and mucous. We previously showed that conjunctival goblet cells express the receptors for RvD1 and that exogenous addition of RvD1 blocks mucin secretion stimulated by LTD 4, LTE 4, and histamine in both rat.
Ogata H, Inoue N, Podolsky DK. Identification of a goblet cell-specific enhancer element in the rat intestinal trefoil factor gene promoter bound by a goblet cell nuclear protein. Biol. Chem., () – PubMed CrossRef Google Scholar. laevis and xP is expected to be a secretory product of intestinal goblet cells.
Based on its strikingly similar distribution, xP might be considered to be the functional X. laevis homolog of TFF2; it is also N-glycosylated as human TFF2.
On a structural basis, xP is unique and represents a. Goblet Cells. The talent of goblet cells is to secrete mucus, a viscous fluid composed primarily of highly glycosylated proteins called mucins suspended in a solution of electrolytes.
Mucus serves many functions, including protection against shear stress and chemical damage, and, especially in the respiratory tree, trapping and elimination of particulate matter and microorganisms. Fine structure of the goblet cell mucous secretory process and subsequent rupturing of the microvilli plasma membrane with discharge of the mucin.
The density of the well developed goblet cell is a function of nuclear and cytoplasmic compression by the mass of mucigen. The fine structure of the “resting goblet” cell is indistinguishable. Conjunctival tissue from rat, mouse, rabbit, and human were excised, fixed in 4% paraformaldehyde, and incubated either with rhodamine-labeled phalloidin to label filamentous actin or in combination with fluoresceinated lectins to label carbohydrates on goblet cell mucins.
The observations are consonant with the belief that mucin is elaborated in the region of the Golgi complex. The cellular structures of epithelial cells both in the normal and inflamed colon are described and the strong osmiophilia of the goblet cell is recorded. Lora V. Hooper, in Advances in Immunology, Goblet cells.
Goblet cells are a secretory epithelial cell lineage found in both the small and the large intestines. A major function of goblet cells is the production of mucus, which forms a protective gel-like layer over the surface epithelium and protects against bacterial invasion (Johansson et al., ) (discussed in detail in Section 4).
Goblet cells are mostly found scattered in the epithelia of the small intestines and respiratory tract. The morphology of goblet cells reflects their function, with the cell containing all the organelles necessary for the production of glycosylated proteins called mucins.
In the respiratory tract, mucins play an important role in catching large particles inhaled by air. Yet, high protein diet consumption induces goblet cell hyperplasia and greater intestinal mucus content in ileum, and modifies goblet cell distribution in rat colonic epithelium (Lan et al., ).
However, since AA, like threonine, are precursors for mucin synthesis. Monospecific antibodies prepared against the rat ,Mr component established its tissue localization in intestinal goblet cells.
Mucins subjected to SDS/polyacrylamide-gel electrophoresis and Western blots using the same antibody, established that the link components of rat and human intestinal mucin are similar antigenically.
Goblet cell carcinoid (GCC) is a rare tumor normally occurring in the appendix which displays features of both a neuroendocrine tumor and a more aggressive form of cancer known as an adenocarcinoma.
  It is usually diagnosed in people over the age of Origin & Development of Goble Cells. Goblet cells along with other principal cells in the gastrointestinal tract, (i.e.
enteroendocrine cells, enterocytes, and Paneth cells), emerge from the multipotent cells (cells that can give rise to different cell types) in the base of the Crypts of Lieberkühn.
In humans, these in general appear during the fetal development of the small intestine at the. Mucin 2 (MUC2), the predominant structural component of the intestinal mucus layer, is exclusively and abundantly expressed by goblet cells in the colon (Tytgat et al., ; Garg et al., Goblet cell fine structure Examination of intestinal goblet cells of man, rat, and guinea pig, after stimulation of the cells to evacuate their mucous, also reveals a basic fine structure that clearly is distinguishable from the fine structure of columnar absorbing cells.
Changes in goblet cell response and mucin production are observed in many intestinal infections caused by parasites, bacteria, and viruses.
Hyperplasia of mucin-secreting goblet cells has been described in a number of helminth parasitic infections including. FIGS. 5A, 5B, 5C, and 5D depict Lectin histochemistry confirming that rat conjunctival goblet cells were associated with UEA-1, which recognizes the l-fucose moiety of glycoproteins in the secretory granules of goblet cells (FIG.
5A) and the secretory product in goblet cells located in the conjuntiva (FIG. 5B). In addition, both primary culture. Many features of the goblet cell that could not be deduced from single planes of section were revealed.
The cell is columnar in configuration. The cytoplasm contains not one, but several discrete clusters of mucin granules, each associated with a separate Golgi apparatus. a General schematic representation of mucus secretion from goblet cells and submucosal glands.
The proposed structure of MUC5AC (threads in dark green) and MUC5B (bundles/strands in bright green) is shown. Mucociliary transport (MCT) of inhaled pathogens and particles (orange spheres of different sizes) is shown with a blue arrow. Goblet cell mucin (GCM) has been purified for the first time from mucosal scrapings of human small intestine.
Proteolytic enzymes and organic solvents were avoided during the isolation procedure. The mucin was purified by Sepharose 4B and 2B column chromatography of. The stomach is a key part of the gastrointestinal (GI) tract, sitting between the esophagus and functions are to mix food with stomach acid and break food down into smaller particles using chemical and mechanical digestion.
The stomach can perform these roles due to the layers of the stomach are the gastric mucosa, submucosa, muscularis externa and serosa. Mantle M, Mantle D, Allen A.
Polymeric structure of pig small-intestinal mucus glycoprotein. Dissociation by proteolysis or by reduction of disulphide bridges. Biochem J. Apr 1; (1)– [PMC free article] Mantle M, Forstner GG, Forstner JF. Antigenic and structural features of goblet-cell mucin of human small intestine.
Human mucin MUC3 and rodent Muc3 are widely assumed to represent secretory mucins expressed in columnar and goblet cells of the intestine. Using a 3′-oligonucleotide probe and in situ hybridization, we observed expression of rat Muc3 mostly in columnar cells.
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Goblet cells are simple columnar goblet shaped like epithelial cells that secrete gel-forming mucins, like mucin MUC5AC. The goblet cells mainly use the merocrine method of secretion, secreting vesicles into a duct, but may use apocrine methods, budding off their secretions, when under stress.
The term goblet refers to the cell's goblet-like shape. The apical portion is shaped like a cup, as. Figure 1 Pic induces MUC5AC rapid secretion directly on goblet cell-like, independent of its serine protease motif.(A, B) Pic degradation avoids detection of mucin hypersecretion.
LST cells were stimulated with different concentrations of Pic (A) or PicSI (B) at 37°C for 4 h. Deoxycholic acid (DCA, mM) was used as a positive control, a known mucin secretagogue on LST cells. SPOC1 cells, which are a mucin-secreting model of rat airway goblet cells, possess a luminal P2Y2 purinoceptor through which UTP, ATP, and ATPgammaS stimulate secretion with EC50 values of.A monoclonal antibody (MAb), designated RGM11, was generated against mucin purified from the surface epithelial layer of rat gastric mucosa.
RGM11 reacted with the purified mucin which had been attached to the ELISA well.this study was directed at identifying the cellular origin of rat intestinal mucin Muc3, the subcellular localization of the Muc3 translation product(s), and alterations in the distribution and forms of intestinal Muc3 in a mouse model of human cystic fibrosis (CF).
The major mucins produced in the rodent intestine are Muc2, a goblet cell product, and Muc3.